Journal: The Journal of Experimental Medicine

Article Title: Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis

doi: 10.1084/jem.20240109

Figure Lengend Snippet: A potentially protective microbiome does not explain DSS resistance of FLNA R mice. (A and B) Pearson correlation analyses of ASV_1lf_am9 (belonging to the genus Lachnospiraceae NK4A136 group) and butyrate concentrations (A) or colitis score (B). (C) CFU determination after plating serial dilutions of fecal pellet slurries ( n = 4 for control and antibiotic [Abx]-treated mice). (D) Fecal microbial loads of control ( n = 4 for FLNA Q , n = 3 for FLNA R ) and antibiotic-treated (Abx) mice (open circles , n = 3) as assessed by flow cytometry (Welch two sample t test). (E) Family level relative abundance profiles of gut microbiomes from FLNA R and FLNA Q control or Abx-treated mice. Families with relative abundances <2% are collapsed into the category Other. Each bar represents one mouse. (F) Alpha diversity metric (Shannon Index) of samples from FLNA Q and FLNA R control and Abx-treated mice, as assessed by 16S rRNA gene amplicon sequencing analyses ( n = 7 for Q Abx; n = 8 for Q control, R control, and R Abx; Welch two sample t test). In D and E, each point represents one mouse, and boxes represent median, first, and third quartiles. Whiskers extend to the highest and lowest values that are within one and a half times the interquartile range. ****P < 0.0001; ***P < 0.001. Data are derived from one experiment.

Article Snippet: 5′-GGACTACNVGGGTWTCTAAT-3′ , MicrosynthAG , 16S RNA barcoding 806R.

Techniques: Control, Flow Cytometry, Amplification, Sequencing, Derivative Assay